As with the previous experiments (Fig 1A), no significant differences were detected in the survival of ΔbcnB compared to wild_type (Fig 1B). The ΔbcnABΔlcoA recovered similar growth levels as wild_type only when complemented with pBcnA, while complementation with pLcoA afforded partial restoration of growth (note that the difference in bacterial recovery between ΔbcnABΔlcoA and ΔbcnABΔlcoA(pLcoA) was statistically significant p=0.0078, Fig 1B). future: Studies are required to elucidate the molecular mechanism of BcnA LcoA and PsrA action and to identify additional protein components involved in the control of peroxidation in the bacterial cell envelope.The analysis was based on determining the average gray values of each bacterial cell where the sum of gray values is divided by the number of pixels. For cloning experiments the authors used HotStar HiFidelity polymerase (QIAGEN). how: Similar results were obtained with the single u0394lcoA and u0394psrA mutants (p and amplt 0.0001 and p and amplt 0.001 respectively).The analysis was performed by MultiGeneBlast as described in Materials and methods. cenocepacia to chemical and physical stress cues that lead to membrane lipid peroxidation. The combined results of all the experiments described above argue that BcnA, LcoA, and PsrA are involved in the adaptation of B.
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